Investigation of 5' messenger RNA stability determinants in lacZ;
citation_date
1994-03
Description
A series of initiation codon and Shine-Dalgarno (SD) sequence changes shows that the SD but not the initiation codon is necessary to maintain lacZ mRNA stability in Escherichia coli. A point mutation in the 4th codon (transcript base 55) of lacZ decreases mRNA stability and beta-galactosidase activity. This mutation also causes decreased beta-galactosidase activity in the background of altered SD and initiation codons. However, mRNA stability is not decreased in the presence of base 55-C and altered initiation codons. Downstream lacZ sequence up to codon 62 is also important for base 55-C's effect on gene expression. Therefore, ribosomal binding but not translation of the coding region is thought to be required for lacZ mRNA stability.
Type
text;
citation_publisher
University of Utah;
citation_keywords
Bacterial Genetics; Proteins;
Subject (MESH)
RNA, Messenger; Escherichia coli;
citation_dissertation_institution
University of Utah;
citation_dissertation_name
PhD
citation_language
en;
Relation-Is Version Of
Digital reproduction of “An investigation of 5' messenger RNA stability determinants in lacZ.” Spencer S. Eccles Health Sciences Library. Print version of “An investigation of 5' messenger RNA stability determinants in lacZ.” available at J. Willard Marriott Library Special Collection. QP6.5 1994 .W33.
