Reactions of carbon fourteen-benzyl-penicillin and carbon fourteen-benzyl-penicilloic acid with human red blood cell ghosts

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Publication Type thesis
School or College School of Medicine
Department Biochemistry
Author Price, Neil Hamon
Contributor Astill, Mark
Title Reactions of carbon fourteen-benzyl-penicillin and carbon fourteen-benzyl-penicilloic acid with human red blood cell ghosts
Date 1978-12
Description Both [14C]-benzylpenicillin and [14C]-benzylpenicilloic acid reacated rapidly with human red blood cell ghosts (FRBCG)1 and human fibroblasts in isotonic phosphate buffer at 37? C. Of the total label bound to FRBCG exposed to either radioactive compound, approximately 40% could be removed by treating the labeled (FRBCG) with various thiols or neutral hydroxylamine. Treatment of (FRBCG) with thiols prior to their exposure to either radioactive compound decreased binding. Exposure of either [14C]-benzylpenicillin and [14C]-benzylpenicilloic acid to sulfhydryl-specific reagents also reduced binding substantially. The data are consistent with they hypothesis that rearrangement products of [14C]-benzylpenicillin and [14C]-benzylpenicilloic acid react with human proteins in a thiol-disulfide interchange type reaction. The rapid reaction of solutions of benzylpenicillin with model disulfides in a thiol-disulfide interchange reaction support this hypothesis.
Type Text
Publisher University of Utah
Subject Disulfide; Fibroblasts
Subject MESH Penicillins; Blood Cells
Dissertation Institution University of Utah
Dissertation Name MS
Language eng
Relation is Version of Digital reproduction of "Reactions of carbon fourteen-benzyl-penicillin and carbon fourteen-benzyl-penicilloic acid with human red blood cell ghosts". Spencer S. Eccles Health Sciences Library. Print version of "Reactions of carbon fourteen-benzyl-penicillin and carbon fourteen-benzyl-penicilloic acid with human red blood cell ghosts" available at J. Willard Marriott Library Special Collection. QR 6.5 1978 P75.
Rights Management © Neil Hamon Price.
Format Medium application/pdf
Format Extent 978,292 bytes
Identifier undthes,4026
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available)
Master File Extent 978,317 bytes
ARK ark:/87278/s6vd719h
Setname ir_etd
ID 191413
Reference URL https://collections.lib.utah.edu/ark:/87278/s6vd719h