Synthesis of fluorinated analogs of oxidative DNA lesions and their use to probe features of recognition and repair by base excision repair glycosylases
| Publication Type | dissertation |
| School or College | College of Science |
| Department | Chemistry |
| Author | Cao, Sheng |
| Title | Synthesis of fluorinated analogs of oxidative DNA lesions and their use to probe features of recognition and repair by base excision repair glycosylases |
| Date | 2010 |
| Description | Damage to DNA occurs through various sources, both exogenous and endogenous. Base lesions resulting from the oxidation of guanine include 8-oxo-7,8-dihydroguanine (OG), guanidinohydantoin (Gh), and spiroiminodihydantoin (Sp). These modified bases can form mismatches and lead to a G - C and G - T transversion mutations in DNA. Fortunately, these lesions are recognized and removed by DNA repair glycosylases found in all organisms. This work explores the features of recognition and repair of these oxidized guanine lesions by a diverse set of base excision glycosylases (MutY, Fpg, hOGG1, Nei, hNEIL1) using synthesized 2' fluorinated nucleosides such as 2'-fluoro-adenosine (FA), 2'-fluoro-8-oxo-guanosine (FOG), 2'-fluoro-guanidinohydan-toin (FGh), and 2'-fluoro-spiroiminodihydantoin (FSp). |
| Type | Text |
| Publisher | University of Utah |
| Subject | DNA lesions; Base excision repair; Glycosylases |
| Subject LCSH | DNA repair; DNA ligases; Molecular recognition |
| Dissertation Institution | University of Utah |
| Dissertation Name | PhD |
| Language | eng |
| Rights Management | ©Sheng Cao |
| Format Extent | 4,827,331 bytes |
| Source | Original in Marriott Library Special Collection, QP6.5 2010 .C26 |
| ARK | ark:/87278/s6qv422w |
| Setname | ir_etd |
| ID | 193185 |
| Reference URL | https://collections.lib.utah.edu/ark:/87278/s6qv422w |