| Publication Type |
thesis |
| School or College |
School of Medicine |
| Department |
Pathology |
| Author |
Slhokoohi, Vida. |
| Title |
Identification and characterizations of gonococci type 5: one step forward in understanding the attachment of gonococci to mucosal cells. |
| Date |
1986-12 |
| Description |
The characteristic of the organisms from gonococcal type 5 morphology and their interaction with human epithelial cells are least studied among different types of gonococci. Organisms from type 5 morphology which were isolated from the fallopian tube of a woman with gonococcal salpingitis exhibited very intense opacity without piliation. After 48 hours of incubation at 37°C with a 2% CO2 environment, organisms from type 5 morphology transformed to type 4 morphology. SDS-PAGE of whole cell lysates of organisms from colonial type 5 revealed presence of a 27kd protein molecule which was absent in the whole cell lysates of the organisms with type 4 morphology, which were obtained as results of colonial type 5 passage. Also silver-stained SDS-PAGE profiles of pronase-treated whole cell lysates of gonococci showed some or all higher peaks in lanes filled with type 4 gonococci than did lanes with type 5 gonococci. This observation suggests that type 4 gonococci may have more lipooligosaccharide or a different form of these molecules than do type 5 gonococci. Attachment to buccal cells of gonococci organisms of colony type 1,4 and 5 differed markedly with the following order: 1>5>4. Colony type 5 gonococci were not piliated, nor were type 4 colonies, so some factor other than pili in type 5 is responsible for the greater attachment of type 5. Since pili are the only attachment factor identified to date, pursuit of the attachment factor used by type 5 gonococci seems warranted. |
| Type |
Text |
| Publisher |
University of Utah |
| Subject |
Pathology; Pathogenicity |
| Subject MESH |
Neisseria gonorrhoeae; Mucous Membrane; Gonorrhea |
| Dissertation Institution |
University of Utah |
| Dissertation Name |
MS |
| Language |
eng |
| Relation is Version of |
Digital reproduction of "Identification and characterizations of gonococci type 5: one step forward in understanding the attachment of gonococci to mucosal cells." Spencer S. Eccles Health Sciences Library. Print version of "Identification and characterizations of gonococci type 5: one step forward in understanding the attachment of gonococci to mucosal cells." available at J. Willard Marriott Library Special Collection. QR 6.5 1986 S54. |
| Rights Management |
© Vida Shokoohi. |
| Format Medium |
application/pdf |
| Identifier |
us-etd2,40 |
| Source |
Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available). |
| ARK |
ark:/87278/s6pg26b8 |
| Setname |
ir_etd |
| ID |
193464 |
| Reference URL |
https://collections.lib.utah.edu/ark:/87278/s6pg26b8 |
