The effect of cortisol on bone cell differentiation.

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Publication Type dissertation
School or College School of Medicine
Department Neurobiology & Anatomy
Author Schafer, Susan Ann.
Contributor Woodbury, Lowell.
Title The effect of cortisol on bone cell differentiation.
Date 1971-08
Description The literature contains inconsistencies concerning the effect of corticosteroids on skeletal tissues. An in vitro system was developed to isolate the effects of cortisol on bone cell populations from those effects due to a secondary hyperparathyroidism. One hour prior to sacrifice, newborn mice were injected with tritiated-thymidine. The radii were placed into organ culture for four days. Effects of cortisol (0.0001, 0.01, 1.0 and 100.0 ug/ml media) and parathyroid extract (0.1, 1.0 and 10.0 units/ml media) on the bone cell populations were studied. Low doses of cortisol (0.0001 ug/ml media) stimulate progenitor cell mitosis and induce bone remodeling through increased differentiation of osteoblasts and osteoclasts. Higher doses of cortisol (above 1.0 ug/ml media) decreased progenitor cell mitosis, inhibit bone formation through decreased osteoblasts differentiation and preferentially protect and increase the resorbing cell population. Parathyroid extract, except at high levels (10 units PTE/ml media), stimulates progenitor cell mitosis and preferentially increases resorbing cell differentiation to the exclusion of osteoblast production. Cortisol and parathyroid extract can be seen to oppose each other in most effects on bone cell populations. While both increase resorbing cell populations, 45Ca metabolism of the whole bone in culture indicates that the activity of the individual cell is depressed by cortisol. Possible inter-relationships of the different bone cells through differentiation and modulation are discussed.
Type Text
Publisher University of Utah
Subject Anatomy; Physiology
Subject MESH Hydrocortisone; Bone Development; Cell Differentiation
Dissertation Institution University of Utah
Dissertation Name PhD
Language eng
Relation is Version of Digital reproduction of "The effect of cortisol on bone cell differentiation." Spencer S. Eccles Health Sciences Library. Print version of "The effect of cortisol on bone cell differentiation." available at J. Willard Marriott Library Special Collection. QP6.5 1971 .S3.
Rights Management © Susan Ann Schafer.
Format Medium application/pdf
Identifier us-etd2,98
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
ARK ark:/87278/s64t7014
Setname ir_etd
ID 193800
Reference URL https://collections.lib.utah.edu/ark:/87278/s64t7014