Poly (ADP-ribosylation) in Vitro: reaction parameters and enzyme mechanism
The reaction catalyzed by poly(ADP-ribose) synthetase from bovine thymus was investigated using the purified enzyme, calf thymus DNA, and histone H1. The rate and extent of poly(ADP-ribosylation), as well as the spectrum of acceptor proteins, changed as reaction conditions were varied. The ionic composition of the buffer greatly influenced the poly(ADP-ribose) synthetase reaction.
American Society for Biochemistry and Molecular Biology (ASBMB)