Aberrant water homeostasis detected by stable isotope analysis
citation_date
2010-07-21
Description
While isotopes are frequently used as tracers in investigations of disease physiology (i.e., 14C labeled glucose), few studies have examined the impact that disease, and disease-related alterations in metabolism, may have on stable isotope ratios at natural abundance levels. The isotopic composition of body water is heavily influenced by water metabolism and dietary patterns and may provide a platform for disease detection. By utilizing a model of streptozotocin (STZ)-induced diabetes as an index case of aberrant water homeostasis, we demonstrate that untreated diabetes mellitus results in distinct combinations, or signatures, of the hydrogen (d2H) and oxygen (d18O) isotope ratios in body water. Additionally, we show that the d2H and d18O values of body water are correlated with increased water flux, suggesting altered blood osmolality, due to hyperglycemia, as the mechanism behind this correlation. Further, we present a mathematical model describing the impact of water flux on the isotopic composition of body water and compare model predicted values with actual values. These data highlight the importance of factors such as water flux and energy expenditure on predictive models of body water and additionally provide a framework for using naturally occurring stable isotope ratios to monitor diseases that impact water homeostasis.
Type
text;
citation_publisher
Public Library of Science (PLoS)
citation_journal_title
PLoS ONE
citation_volume
5
citation_issue
7
citation_firstpage
e11699
Citation_lastpage
7
citation_doi
10.1371/journal.pone.0011699
citatation_issn
1932-6203
citation_language
eng;
Bibliographic Citation
OGrady, S. P., Wende, A. R., Remien, C. H., Valenzuela, L. O., Enright, L. E., Chesson, L. A., Abel, E. D., Cerling, T. E., & Ehleringer, J. R. (2010). Aberrant water homeostasis detected by stable isotope analysis. PLoS ONE, 5(7), 1-7.
Rights Management
(c) Ehleringer, J. R. (2010). Aberrant water homeostasis detected by stable isotope analysis. PLoS ONE, 5 (7), 1-7.
