School of Medicine; College of Science; College of Engineering
Human Genetics; Howard Hughes Medical Institute; Bioengineering; Biology
Gesteland, Raymond F.; Atkins, John F.; Tuohy, Therese M.
Uninterrupted translation through putative 12-nucleotide coding gap in sequence of carA: business as usual
Previous work of others reported an untranslated stretch of 12 nucleotides in the 5' coding sequence of carA from Pseudomonas aeruginosa. However, N-terminal protein sequencing of carA-lacZ translational fusions shows that these 12 nucleotides are normally translated in a continuous triplet manner, both in P. aeruginosa and in Escherichia coli.
American Society for Microbiology
Base Sequence; Protein Biosynthesis; Sequence Deletion;